topflash fopflash reporter Search Results


topflash/fopflash wnt/-catenin signaling reporter  (Beyotime)
90
Beyotime topflash/fopflash wnt/-catenin signaling reporter
Topflash/Fopflash Wnt/ Catenin Signaling Reporter, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash/fopflash wnt/-catenin signaling reporter/product/Beyotime
Average 90 stars, based on 1 article reviews
topflash/fopflash wnt/-catenin signaling reporter - by Bioz Stars, 2026-03
90/100 stars
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topflash/fopflash tcf reporter kit  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc topflash/fopflash tcf reporter kit
Topflash/Fopflash Tcf Reporter Kit, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash/fopflash tcf reporter kit/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
topflash/fopflash tcf reporter kit - by Bioz Stars, 2026-03
90/100 stars
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reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites
Reporter Plasmids Containing Wild Type (Cctttgatc; Top Flash) Or Mutated (Cctttggcc; Fop Flash) Tcf/Lef Dna Binding Sites, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites - by Bioz Stars, 2026-03
90/100 stars
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wnt/β-catenin signaling reporter topflash/fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc wnt/β-catenin signaling reporter topflash/fopflash
Wnt/β Catenin Signaling Reporter Topflash/Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt/β-catenin signaling reporter topflash/fopflash/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
wnt/β-catenin signaling reporter topflash/fopflash - by Bioz Stars, 2026-03
90/100 stars
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topflash and fopflash luciferase reporter  (Promega)
90
Promega topflash and fopflash luciferase reporter
Sfrp1 inhibits Wnt7a activity in the <t>TOPflash</t> luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 <t>and</t> <t>pcDNA3.1-Dkk1</t> , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.
Topflash And Fopflash Luciferase Reporter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash and fopflash luciferase reporter/product/Promega
Average 90 stars, based on 1 article reviews
topflash and fopflash luciferase reporter - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
topflash/fopflash reporters  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc topflash/fopflash reporters
miR-324-3p promoted the growth of gastric organoids and activated the Wnt/beta-catenin signaling pathway via downregulation of Smad4. a Photo of gastric organoids we constructed. b HE staining of gastric organoids. c The expression of miR-324-3p in gastric organoids was upregulated by miR-324-3p mimics lentivirus. d miR-324-3p could increase the size of gastric organoids. e The results of ki-67 staining showed that miR-324-3p had a positive effect on growth of gastric organoids. f The results of <t>TOPflash/FOPflash</t> showed that the Wnt/beta-catenin signaling pathway was activated by overexpression of miR-324-3p and knockdown of miR-324-3p inhibited the Wnt/beta-catenin signaling pathway. g Overexpression of miR-324-3p increased the protein expression level of beta-catenin and Wnt-dependent cyclin D1, CD44, c-Met, c-Jun, and TCF-1 while inhibition of miR-324-3p had the opposite effect. h Overexpression of Smad4 downregulated the expression level of beta-catenin, cyclin D1, CD44, c-Met, c-Jun, and TCF-1. i Knockdown of miR-324-3p reduced intracellular ATP level in the BGC-823 cell line. miR-324-3p increased intracellular ATP level in the MGC-803 cell line and Smad4 could reverse the effect of miR-324-3p on intracellular ATP level. j The intracellular ATP levels of GC cells under hypoxic conditions
Topflash/Fopflash Reporters, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash/fopflash reporters/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
topflash/fopflash reporters - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
wnt signaling reporter (topflash or fopflash)  (Promega)
90
Promega wnt signaling reporter (topflash or fopflash)
miR-324-3p promoted the growth of gastric organoids and activated the Wnt/beta-catenin signaling pathway via downregulation of Smad4. a Photo of gastric organoids we constructed. b HE staining of gastric organoids. c The expression of miR-324-3p in gastric organoids was upregulated by miR-324-3p mimics lentivirus. d miR-324-3p could increase the size of gastric organoids. e The results of ki-67 staining showed that miR-324-3p had a positive effect on growth of gastric organoids. f The results of <t>TOPflash/FOPflash</t> showed that the Wnt/beta-catenin signaling pathway was activated by overexpression of miR-324-3p and knockdown of miR-324-3p inhibited the Wnt/beta-catenin signaling pathway. g Overexpression of miR-324-3p increased the protein expression level of beta-catenin and Wnt-dependent cyclin D1, CD44, c-Met, c-Jun, and TCF-1 while inhibition of miR-324-3p had the opposite effect. h Overexpression of Smad4 downregulated the expression level of beta-catenin, cyclin D1, CD44, c-Met, c-Jun, and TCF-1. i Knockdown of miR-324-3p reduced intracellular ATP level in the BGC-823 cell line. miR-324-3p increased intracellular ATP level in the MGC-803 cell line and Smad4 could reverse the effect of miR-324-3p on intracellular ATP level. j The intracellular ATP levels of GC cells under hypoxic conditions
Wnt Signaling Reporter (Topflash Or Fopflash), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wnt signaling reporter (topflash or fopflash)/product/Promega
Average 90 stars, based on 1 article reviews
wnt signaling reporter (topflash or fopflash) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway  (Merck KGaA)
90
Merck KGaA topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway
miR-324-3p promoted the growth of gastric organoids and activated the Wnt/beta-catenin signaling pathway via downregulation of Smad4. a Photo of gastric organoids we constructed. b HE staining of gastric organoids. c The expression of miR-324-3p in gastric organoids was upregulated by miR-324-3p mimics lentivirus. d miR-324-3p could increase the size of gastric organoids. e The results of ki-67 staining showed that miR-324-3p had a positive effect on growth of gastric organoids. f The results of <t>TOPflash/FOPflash</t> showed that the Wnt/beta-catenin signaling pathway was activated by overexpression of miR-324-3p and knockdown of miR-324-3p inhibited the Wnt/beta-catenin signaling pathway. g Overexpression of miR-324-3p increased the protein expression level of beta-catenin and Wnt-dependent cyclin D1, CD44, c-Met, c-Jun, and TCF-1 while inhibition of miR-324-3p had the opposite effect. h Overexpression of Smad4 downregulated the expression level of beta-catenin, cyclin D1, CD44, c-Met, c-Jun, and TCF-1. i Knockdown of miR-324-3p reduced intracellular ATP level in the BGC-823 cell line. miR-324-3p increased intracellular ATP level in the MGC-803 cell line and Smad4 could reverse the effect of miR-324-3p on intracellular ATP level. j The intracellular ATP levels of GC cells under hypoxic conditions
Topflash/Fopflash Reporter Constructs For Measuring Tcf/Lef Activity In The Beta Catenin Signaling Pathway, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway/product/Merck KGaA
Average 90 stars, based on 1 article reviews
topflash/fopflash reporter constructs for measuring tcf/lef activity in the beta-catenin signaling pathway - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Journal: Frontiers in Molecular Neuroscience

Article Title: Opposite Roles of Wnt7a and Sfrp1 in Modulating Proper Development of Neural Progenitors in the Mouse Cerebral Cortex

doi: 10.3389/fnmol.2018.00247

Figure Lengend Snippet: Sfrp1 inhibits Wnt7a activity in the TOPflash luciferase reporter assay. (A) TOPflash is a luciferase reporter of β-catenin-mediated transcriptional activation with active TCF/LEF binding sites, which affect the firefly luciferase expression. The control plasmid is FOPflash , which contains mutant TCF/LEF binding sites. (B,C) After transfection of the pcDNA3.1-Sfrp1 and pcDNA3.1-Dkk1 , a statistically significant decrease in luciferase activity of Wnt1 and Wnt7a was observed in comparison with controls. Values represent mean ± SEM. n = 3, ∗∗ P < 0.01; ∗∗∗ P < 0.001; unpaired Student’s t -test.

Article Snippet: The Sfrp1 , Dkk1 coding sequences were subcloned into the pcDNA3.1 vector for the TOPflash and FOPflash luciferase reporter (Promega, United States) assay.

Techniques: Activity Assay, Luciferase, Reporter Assay, Activation Assay, Binding Assay, Expressing, Control, Plasmid Preparation, Mutagenesis, Transfection, Comparison

miR-324-3p promoted the growth of gastric organoids and activated the Wnt/beta-catenin signaling pathway via downregulation of Smad4. a Photo of gastric organoids we constructed. b HE staining of gastric organoids. c The expression of miR-324-3p in gastric organoids was upregulated by miR-324-3p mimics lentivirus. d miR-324-3p could increase the size of gastric organoids. e The results of ki-67 staining showed that miR-324-3p had a positive effect on growth of gastric organoids. f The results of TOPflash/FOPflash showed that the Wnt/beta-catenin signaling pathway was activated by overexpression of miR-324-3p and knockdown of miR-324-3p inhibited the Wnt/beta-catenin signaling pathway. g Overexpression of miR-324-3p increased the protein expression level of beta-catenin and Wnt-dependent cyclin D1, CD44, c-Met, c-Jun, and TCF-1 while inhibition of miR-324-3p had the opposite effect. h Overexpression of Smad4 downregulated the expression level of beta-catenin, cyclin D1, CD44, c-Met, c-Jun, and TCF-1. i Knockdown of miR-324-3p reduced intracellular ATP level in the BGC-823 cell line. miR-324-3p increased intracellular ATP level in the MGC-803 cell line and Smad4 could reverse the effect of miR-324-3p on intracellular ATP level. j The intracellular ATP levels of GC cells under hypoxic conditions

Journal: Journal of Gastroenterology

Article Title: miR-324-3p promotes gastric cancer development by activating Smad4-mediated Wnt/beta-catenin signaling pathway

doi: 10.1007/s00535-017-1408-0

Figure Lengend Snippet: miR-324-3p promoted the growth of gastric organoids and activated the Wnt/beta-catenin signaling pathway via downregulation of Smad4. a Photo of gastric organoids we constructed. b HE staining of gastric organoids. c The expression of miR-324-3p in gastric organoids was upregulated by miR-324-3p mimics lentivirus. d miR-324-3p could increase the size of gastric organoids. e The results of ki-67 staining showed that miR-324-3p had a positive effect on growth of gastric organoids. f The results of TOPflash/FOPflash showed that the Wnt/beta-catenin signaling pathway was activated by overexpression of miR-324-3p and knockdown of miR-324-3p inhibited the Wnt/beta-catenin signaling pathway. g Overexpression of miR-324-3p increased the protein expression level of beta-catenin and Wnt-dependent cyclin D1, CD44, c-Met, c-Jun, and TCF-1 while inhibition of miR-324-3p had the opposite effect. h Overexpression of Smad4 downregulated the expression level of beta-catenin, cyclin D1, CD44, c-Met, c-Jun, and TCF-1. i Knockdown of miR-324-3p reduced intracellular ATP level in the BGC-823 cell line. miR-324-3p increased intracellular ATP level in the MGC-803 cell line and Smad4 could reverse the effect of miR-324-3p on intracellular ATP level. j The intracellular ATP levels of GC cells under hypoxic conditions

Article Snippet: TOPflash/FOPflash reporters were purchased from Upstate Biotechnology Inc (Lake Placid, NY, USA).

Techniques: Construct, Staining, Expressing, Over Expression, Inhibition